Mouse monoclonal (TB21) antibody to Transforming Growth Factor beta.
Specificity
Western blotting demonstrated that this antibody reacts with the dimeric (25 kDa) and monomeric (12.5 kDa) forms of TGF-b under both non-reducing and reducing conditions respectively. This antibody recognises both human platelet-derived and recombinant TGF-b in ELISA.
Applications
IHC, WB
Reactivity
Human
Immunogen
TGF-β from human platelets.
Host
Mouse
Clonality
Monoclonal
Clone ID
TB21
Myeloma
Sp2/0-Ag14
Isotype
IgG1
Conjugate
Unconjugated
Concentration
Reconstitution dependent.
Purity
Protein G affinity chromatagraphy.
Product Form
Lyophilised
Reconstitution
Reconstitute with double distilled water.
Formulation
Lyophilised from a solution of 0.01M Phosphate Buffered Saline, pH 7.0.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Western Blot - Anti-TGF beta Antibody [TB21] (A32625)
Western blot result of Anti-TGF beta Antibody (2 mg/ml) used to detect platelet derived TGF-b at: 12 mg/lane (Lane 2), 6 mg/lane (Lane 3), 3 mg/lane (Lane 4) and 1 mg/lane (Lane 5) with 4-chlorol-napthol as substrate. The bottom band is the 12.5KD TGF-b monomer, the mid-band is the 25KD TGF-b dimer and the up band is TGF-b with signal peptide. Lane 1 is molecular weight markers.
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