Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
CXXC-type zinc finger protein 6, CXXC6, KIAA1676, LCX, Leukemia-associated protein with a CXXC domain, Methylcytosine dioxygenase TET1, Ten-eleven translocation 1 gene protein
Western blot analysis of extracts of various cell lines, using Anti-TET1 Antibody (A13475) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded mouse fetal Brain using Anti-TET1 Antibody (A13475) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of HeLa cells using Anti-TET1 Antibody (A13475) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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