Anti-TCR alpha + TCR beta Antibody [R73] (A281640)

$350

Mouse monoclonal [R73] antibody to TCR alpha + TCR beta for IP, Flow Cytometry and IHC-Fr.

Alternative Formats and ConjugatesAlternative Formats

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-7 business days.
Name
Anti-TCR alpha + TCR beta Antibody [R73]
Description
Mouse monoclonal [R73] antibody to TCR alpha + TCR beta.
Specificity
This antibody recognises a constant determinant on the beta chain of the rat alpha/beta T cell receptor, expressed by 97% of peripheral rat T cells as defined by the OX-52 marker. R73 is mitogenic for unseparated spleen cells and for purified T cells. In the rat thymus, mature medullary cells express the R73 determinant at the same levels as peripheral T cells, whereas 94% of CD4 - CD8 - thymocytes are R73 negative. Mouse anti Rat TCR alpha/beta antibody, clone R73 is reported to stimulate adhesion between Thymic Dendritic Cells and Thymocytes.
Applications
IP, Flow Cytometry, IHC-Fr
Dilutions
Flow Cytometry: 1:50 - 1:100, IHC-Fr: 1:10 - 1:100, Use 10µl of the suggested working dilution to label 100µl of whole blood or 106 cells in 100µl.
Reactivity
Rat, Monkey, Cynomolgus monkey
Immunogen
Rat T Blasts and Erythrocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
R73
Isotype
IgG1
Conjugate

Unconjugated

Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Rat TCR alpha/beta antibody, clone R73 recognizes a constant determinant on the beta chain of the rat alpha/beta T cell receptor, expressed by 97% of peripheral rat T cells as defined by the OX-52 marker. R73 is mitogenic for unseparated spleen cells and for purified T cells. In the rat thymus, mature medullary cells express the R73 determinant at the same levels as peripheral T cells, whereas 94% of CD4 - CD8 - thymocytes are R73 negative.Mouse anti Rat TCR alpha/beta antibody, clone R73 is reported to stimulate adhesion between Thymic Dendritic Cells and Thymocytes (Colic et al. 2010).
Synonyms
T cell receptor beta variable 7-9
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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