Anti-TCR alpha + TCR beta Antibody [R73] (A281640)

Anti-TCR alpha + TCR beta Antibody [R73]

Mouse monoclonal [R73] antibody to TCR alpha + TCR beta.

This antibody recognises a constant determinant on the beta chain of the rat alpha/beta T cell receptor, expressed by 97% of peripheral rat T cells as defined by the OX-52 marker. R73 is mitogenic for unseparated spleen cells and for purified T cells. In the rat thymus, mature medullary cells express the R73 determinant at the same levels as peripheral T cells, whereas 94% of CD4 - CD8 - thymocytes are R73 negative. Mouse anti Rat TCR alpha/beta antibody, clone R73 is reported to stimulate adhesion between Thymic Dendritic Cells and Thymocytes.

IP, Flow Cytometry, IHC-Fr

Flow Cytometry: 1:50 - 1:100, IHC-Fr: 1:10 - 1:100, Use 10µl of the suggested working dilution to label 100µl of whole blood or 10⁶ cells in 100µl.

Rat, Monkey, Cynomolgus monkey

Rat T Blasts and Erythrocytes.

Mouse

Monoclonal

R73

IgG1

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Rat TCR alpha/beta antibody, clone R73 recognizes a constant determinant on the beta chain of the rat alpha/beta T cell receptor, expressed by 97% of peripheral rat T cells as defined by the OX-52 marker. R73 is mitogenic for unseparated spleen cells and for purified T cells. In the rat thymus, mature medullary cells express the R73 determinant at the same levels as peripheral T cells, whereas 94% of CD4 - CD8 - thymocytes are R73 negative.Mouse anti Rat TCR alpha/beta antibody, clone R73 is reported to stimulate adhesion between Thymic Dendritic Cells and Thymocytes (Colic et al. 2010).

T cell receptor beta variable 7-9