Recombinant mouse monoclonal [rACP5/1070] antibody to Tartrate Resistant Acid Phosphatase.
Specificity
This antibody recognizes both isoforms (5a and 5b) of TRAcP. It labels the cells of Hairy Cell Leukemia with a high degree of sensitivity and specificity and also stains tissue macrophages and osteoclasts.
Applications
IHC-P
Dilutions
IHC-P: 1-2 µg/ml
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant full-length human ACP5 protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
rACP5/1070
Isotype
IgG1
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A/G chromatography.
Concentration
1 mg/ml
Molecular Weight
35 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
SDS-PAGE analysis of Anti-Tartrate Resistant Acid Phosphatase Antibody [rACP5/1070] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
Analysis of protein array containing more than 19,000 full-length human proteins using Anti-Tartrate Resistant Acid Phosphatase Antibody [rACP5/1070]. Z-Score and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target; a MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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