Figure 1: Western Blot - Anti-SYVN1/HRD1 Antibody (A82599)
SYVN1/HRD1 expression in Human Cerebellum (A) and Rat Lung (B) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-SYVN1/HRD1 Antibody (A82599) at 2µg/ml and detected by chemiluminescence.
SYVN1/HRD1 expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-SYVN1/HRD1 Antibody (A82599) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. ER and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
SYVN1/HRD1 expression in A431 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-SYVN1/HRD1 Antibody (A82599) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.
