Synthetic nanodiscs offer a stable and biologically relevant environment that closely mimics cell membranes and enables full-length transmembrane human GPCR GPR14 protein to be purified and analysed in vitro.
Sistema di espressione
HEK293 cells
Tipo di proteina
Synthetic
Specie proteiche
Human
Lunghezza
Full length protein.
Peso molecolare
Full length human GPCR GPR14 protein has a MW of 41.9 kDa.
Coniugare
Unconjugated
Forma del prodotto
Lyophilized
Concentrazione
Reconstitution dependent.
Formulazione
Lyophilized from nanodisc solubilization buffer (20mM Tris-HCl, 150mM NaCl, pH 8.0). Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Conservazione
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Sinonimi
G protein coupled receptor 14, G-protein coupled receptor 14, GPR14, UR-2-R, UR-II-R, UR2R_HUMAN, Urotensin II receptor, Urotensin II receptor (UR II R), Urotensin-2 receptor, UTR, UTR2, Uts2r
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Figure 1: Synthetic Nanodisc Human GPCR GPR14 Protein (A317295)
ELISA plates were pre-coated with Synthetic Nanodisc Human GPCR GPR14 Protein (A317295) at 0.2µg/per well. Serial diluted Anti-Flag Monoclonal Antibody solutions were added, washed, and incubated with a secondary antibody before reading the ELISA. From the above data, the EC50 for Anti-Flag Monoclonal Antibody binding with Synthetic Nanodisc Human GPCR GPR14 Protein (A317295) is 10.47ng/ml.
