Synthetic nanodiscs offer a stable and biologically relevant environment that closely mimics cell membranes and enables full-length transmembrane human Frizzled 9 protein to be purified and analysed in vitro.
Sistema di espressione
HEK293 cells
Tipo di proteina
Synthetic
Specie proteiche
Human
Lunghezza
Full length protein.
Peso molecolare
Full length human Frizzled 9 protein has a MW of 64.5 kDa.
Coniugare
Unconjugated
Forma del prodotto
Lyophilized
Concentrazione
Reconstitution dependent.
Formulazione
Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization.
Conservazione
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Figure 1: Synthetic Nanodisc Human Frizzled 9 Protein (A325139)
Diagram showing how synthetic nanodiscs containing full-length multi-pass transmembrane proteins in a phospholipid bilayer are generated from native cell membranes.
