Synthetic nanodiscs offer a stable and biologically relevant environment that closely mimics cell membranes and enables full-length transmembrane human DARC protein to be purified and analysed in vitro.
Sistema di espressione
HEK293 cells
Tipo di proteina
Synthetic
Specie proteiche
Human
Lunghezza
Full length protein.
Peso molecolare
Full length human DARC protein has a MW of 35.6 kDa.
Coniugare
Unconjugated
Forma del prodotto
Lyophilized
Concentrazione
Reconstitution dependent.
Formulazione
Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization.
Conservazione
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Figure 1: Synthetic Nanodisc Human DARC Protein (A325116)
Diagram showing how synthetic nanodiscs containing full-length multi-pass transmembrane proteins in a phospholipid bilayer are generated from native cell membranes.
