Rabbit polyclonal antibody to STAT5 (phospho Tyr694).
Applicazioni
WB
Diluizioni consigliate
WB: 1:500-1:1,000
Reattività
Human
Immunogeno
A synthetic phosphorylated peptide around Y694 of human STAT5A (NP_001275647.1).
Sequenza
DGYVK
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare
Unconjugated
Purificazione
Affinity purification.
Peso molecolare
90 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
MGF, Signal transducer and activator of transcription 5A, Signal Transducer and Activator of Transcription 5B, STA5A_HUMAN, STAT 5A, STAT 5B, STAT5A, STAT5B, Transcription factor STAT5A, Transcription factor STAT5B
Figure 1: Western Blot - Anti-STAT5 (phospho Tyr694) Antibody (A16466)
Western blot analysis of extracts of TF-1 cells, using Anti-STAT5 (phospho Tyr694) Antibody (A16466) at 1:1,000 dilution. TF-1 cells were treated by G-CSF (25 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
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