Anti-STAT3 (phospho Ser727) Anticorpo (A91363)

Name: Anti-STAT3 (phospho Ser727) Antibody
Brand: Antibodies.com
SKU: A91363
Price: 435.0 USD
Availability: InStock

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$435

Anticorpo policlonale di coniglio contro STAT3 (phospho Ser727) per WB, IHC e IP.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-STAT3 (phospho Ser727) Antibody

Descrizione

Rabbit polyclonal antibody to STAT3 (phospho Ser727).

Applicazioni

WB, IHC, IP

Diluizioni consigliate

WB: 1:500-1:1,000, IHC: 1:50-1:200, IP: 1:50-1:100

Reattività

Human, Mouse, Rat

Immunogeno

A synthetic phosphorylated peptide around S727 of human Phospho-STAT3-S727 (NP_644805.1).

Sequenza

PMSPR

Specie ospite

Rabbit

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

86 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

1110034C02Rik, Acute Phase Response Factor, Acute-phase response factor, ADMIO, APRF, AW109958, DNA binding protein APRF, FLJ20882, HIES, MGC16063, Signal transducer and activator of transcription 3, Signal transducer and activator of transcription 3 (acute phase response factor), STAT 3, STAT3_HUMAN

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (6)

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Figure 1: Western Blot - Anti-STAT3 (phospho Ser727) Antibody (A91363)

Western blot analysis of extracts of HeLa cells, using Anti-STAT3 (phospho Ser727) Antibody (A91363) at 1:1,000 dilution. HeLa cells were treated by UV at room temperature for 15-30 minutes. HeLa cells were treated by PMA/TPA (200 nM) at 37°C for 15 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-STAT3 (phospho Ser727) Antibody (A91363)

Western blot analysis of extracts of various cell lines, using Anti-STAT3 (phospho Ser727) Antibody (A91363) at 1:1,000 dilution. HepG2 cells and C6 cells were treated by PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight. HepG2 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 3: Immunohistochemistry - Anti-STAT3 (phospho Ser727) Antibody (A91363)

Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-STAT3 (phospho Ser727) Antibody (A91363) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-STAT3 (phospho Ser727) Antibody (A91363)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-STAT3 (phospho Ser727) Antibody (A91363) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.