Rabbit polyclonal antibody to Src (Phospho-Tyr418)
Specificity
The antibody detects endogenous level of Src only when phosphorylated at tyrosine 418.
Applications
WB, IHC
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of tyrosine 418(N-E-Y(p)-T-A)derived from Human Src.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Predicted MW
60kd
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from COLO205 cells using Src (Ab-418) antibody (#21115, Lane 1 and 2) and Src (phospho-Tyr418) antibody (#11091, Lane 3 and 4).
![Western Blot - Anti-Src Antibody [ARC0378] (A307395) - Antibodies.com](https://cdn.antibodies.com/image/catalog/307/A307395_1.jpg?profile=product_alternative)
![Western Blot - Anti-Src (phospho Tyr416) Antibody [ARC57736] (A309701) - Antibodies.com](https://cdn.antibodies.com/image/catalog/309/A309701_1.jpg?profile=product_alternative)
![Western Blot - Anti-Src (phospho Tyr419) Antibody [ARC1572] (A308034) - Antibodies.com](https://cdn.antibodies.com/image/catalog/308/A308034_1.jpg?profile=product_alternative)
![Western Blot - Anti-Src (phospho Tyr529) Antibody [ARC54665] (A309480) - Antibodies.com](https://cdn.antibodies.com/image/catalog/309/A309480_1.jpg?profile=product_alternative)
