Anti-SPRY1 Antibody (A83695)

SPRY1 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-SPRY1 Antibody (A83695) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 4µg/ml. Cytoplasmic and Golgi apparatus staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 4µg/ml.

SPRY1 expression in Human Kidney lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-SPRY1 Antibody (A83695) at 0.3µg/ml and detected by chemiluminescence.

SPRY1 expression in HepG2 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-SPRY1 Antibody (A83695) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 4µg/ml. Cytoplasmic and Golgi apparatus staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 4µg/ml.

SPRY1 expression in HEK293 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-SPRY1 Antibody (A83695) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 0.4µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.