Anti-Smoothened Antibody (A16839)

Name: Anti-Smoothened Antibody
Brand: Antibodies.com
SKU: A16839
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to Smoothened for WB.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Smoothened Antibody

Description

Rabbit polyclonal antibody to Smoothened.

Applications

Dilutions

WB: 1:100-1:500

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 54-219 of human SMO (NP_005622.1).

Sequence

VTGPPPPLSHCGRAAPCEPLRYNVCLGSVLPYGATSTLLAGDSDSQEEAHGKLVLWSGLRNAPRCWAVIQPLLCAVYMPKCENDRVELPSRTLCQATRGPCAIVERERGWPDFLRCTPDRFPEGCTNEVQNIKFNSSGQCEVPLVRTDNPKSWYEDVEGCGIQCQN

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

86 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Protein Gx, Protein smoothened, SMO, SMOH

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-Smoothened Antibody (A16839)

Western blot analysis of extracts of various cell lines, using Anti-Smoothened Antibody (A16839) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 120s.

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Western Blot - Anti-Smoothened Antibody (A16839)

Western blot analysis of extracts of Mouse brain, using Anti-Smoothened Antibody (A16839) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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