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Anti-Smad3 (phospho Thr179) Antibody (A94164)

Western Blot - Anti-Smad3 (phospho Thr179) Antibody (A1003) - Antibodies.com Immunohistochemistry - Anti-Smad3 (phospho Thr179) Antibody (A1003) - Antibodies.com Western Blot - Anti-Smad3 (phospho Thr179) Antibody (A1003) - Antibodies.com
$190
Product Datasheet

Rabbit polyclonal antibody to Smad3 (phospho Thr179) for WB, IHC and ELISA.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.

  • Specifications

Name
Anti-Smad3 (phospho Thr179) Antibody
Description
Rabbit polyclonal antibody to Smad3 (phospho Thr179).
Specificity
This antibody detects endogenous levels of Smad3 only when phosphorylated at Thr179.
Applications
WB, IHC, ELISA
Dilutions
WB: 1:500-1:1000, IF: 1:100-1:500, ELISA: 1:5000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide derived from human Smad3 around the phosphorylation site of Thr179 (amino acids 145-194).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
48kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
hMAD-3, hSMAD3, JV15-2, MAD homolog 3, Mad3, MADH3, Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMAD 3, SMAD family member 3
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-Smad3 (phospho Thr179) Antibody (A1003) - Antibodies.comEnlarge Image

Western Blot - Anti-Smad3 (phospho Thr179) Antibody (A94164)

Western blot analysis of lysates from HeLa cells treated with TNF 20ng/ml 2' using Anti-Smad3 (phospho Thr179) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunohistochemistry - Anti-Smad3 (phospho Thr179) Antibody (A1003) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-Smad3 (phospho Thr179) Antibody (A94164)

Immunohistochemical analysis of paraffin-embedded human heart using Anti-Smad3 (phospho Thr179) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Western Blot - Anti-Smad3 (phospho Thr179) Antibody (A1003) - Antibodies.comEnlarge Image

Western Blot - Anti-Smad3 (phospho Thr179) Antibody (A94164)

Western blot analysis of HELA cells using Anti-Smad3 (phospho Thr179) Antibody.
Publishing research using Anti-Smad3 (phospho Thr179) Antibody (A94164)? Please let us know so that we can list the citation on this page.

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