Anti-Smad3 (phospho Thr179) Anticorpo (A11032)

Name: Anti-Smad3 (phospho Thr179) Antibody
Brand: Antibodies.com
SKU: A11032
Price: 435.0 USD
Availability: InStock

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$435

Anticorpo policlonale di coniglio contro Smad3 (phospho Thr179) per WB e IHC.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-Smad3 (phospho Thr179) Antibody

Descrizione

Rabbit polyclonal antibody to Smad3 (phospho Thr179).

Applicazioni

WB, IHC

Diluizioni consigliate

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reattività

Human, Mouse, Rat

Immunogeno

A synthetic phosphorylated peptide around T179 of human Smad3 (NP_005893.1).

Sequenza

PETPP

Specie ospite

Rabbit

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

48 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

DKFZP586N0721, DKFZp686J10186, hMAD 3, hMAD-3, hSMAD3, HSPC193, HST17436, JV15 2, JV15-2, JV152, LDS1C, LDS3, MAD (mothers against decapentaplegic Drosophila) homolog 3, MAD homolog 3, Mad homolog JV15 2, Mad protein homolog, MAD, mothers against decapentaplegic homolog 3, Mad3, MADH 3, MADH3, MGC60396, Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA and MAD related protein 3, SMAD, SMAD 3, SMAD family member 3, SMAD, mothers against DPP homolog 3, SMAD3_HUMAN

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Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (5)

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Figure 1: Western Blot - Anti-Smad3 (phospho Thr179) Antibody (A11032)

Western blot analysis of extracts of HeLa cell line, using Anti-Smad3 (phospho Thr179) Antibody (A11032). The lane on the right is treated with the antigen-specific peptide. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA.

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Figure 2: Western Blot - Anti-Smad3 (phospho Thr179) Antibody (A11032)

Western blot analysis of extracts of PC-12 cells, using Anti-Smad3 (phospho Thr179) Antibody (A11032) at 1:1,000 dilution. PC-12 cells were treated by TNF-alpha (20 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Immunohistochemistry - Anti-Smad3 (phospho Thr179) Antibody (A11032)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-Smad3 (phospho Thr179) Antibody (A11032) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Smad3 (phospho Thr179) Antibody (A11032)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Smad3 (phospho Thr179) Antibody (A11032) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.