Western Blot - Anti-Smad3 (phospho Ser425) Antibody (A94165)
Western blot analysis of lysates from NIH/3T3 cells treated with IGF-I 100ng/ml 10' using Anti-Smad3 (phospho Ser425) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-Smad3 (phospho Ser425) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Immunohistochemical analysis of paraffin-embedded human uterus tissue using Anti-Smad3 (phospho Ser425) Antibody at 1:200 (4°C overnight). Negative control was secondary antibody only.
Immunohistochemical analysis of paraffin-embedded mouse lung tissue using Anti-Smad3 (phospho Ser425) Antibody at 1:200 (4°C overnight). Negative control was secondary antibody only.
Immunofluorescence analysis of mouse kidney tissue using Anti-Smad3 (phospho Ser425) Antibody (red) at 1:200 (4°C overnight). Cy3 labelled secondary antibody was used at 1:300 (RT 50min). Panel A: Target. Panel B: DAPI. Panel C: Merge.
Immunofluorescence analysis of rat lung tissue using Anti-Smad3 (phospho Ser425) Antibody (red) at 1:200 (4°C overnight). Cy3 labelled secondary antibody was used at 1:300 (RT 50min). Panel A: Target. Panel B: DAPI. Panel C: Merge.