Anti-Smad3 (phospho Ser423 + Ser425) Anticorpo [ARC0170] (A308811)

Name: Anti-Smad3 (phospho Ser423 + Ser425) Antibody [ARC0170]
Brand: Antibodies.com
SKU: A308811
Price: 540.0 USD
Availability: InStock

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$540

Anticorpo monoclonale [ARC0170] di coniglio contro Smad3 (phospho Ser423 + Ser425) per WB e IHC.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-Smad3 (phospho Ser423 + Ser425) Antibody [ARC0170]

Descrizione

Rabbit monoclonal [ARC0170] antibody to Smad3 (phospho Ser423 + Ser425).

Applicazioni

WB, IHC

Diluizioni consigliate

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reattività

Human, Mouse, Rat

Immunogeno

A synthetic phosphorylated peptide around S423 & S425 of human Smad3 (P84022).

Sequenza

SSVS

Specie ospite

Rabbit

Clonalità

Monoclonal

Clona ID

ARC0170

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

55 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

DKFZP586N0721, DKFZp686J10186, hMAD 3, hMAD-3, hSMAD3, HSPC193, HST17436, JV15 2, JV15-2, JV152, LDS1C, LDS3, MAD (mothers against decapentaplegic Drosophila) homolog 3, MAD homolog 3, Mad homolog JV15 2, Mad protein homolog, MAD, mothers against decapentaplegic homolog 3, Mad3, MADH 3, MADH3, MGC60396, Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA and MAD related protein 3, SMAD, SMAD 3, SMAD family member 3, SMAD, mothers against DPP homolog 3, SMAD3_HUMAN

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Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (4)

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Figure 1: Western Blot - Anti-Smad3 (phospho Ser423 + Ser425) Antibody [ARC0170] (A308811)

Western blot analysis of extracts of C6 cells, using Anti-Smad3 (phospho Ser423 + Ser425) Antibody [ARC0170] (A308811) at 1:1,000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37°C for 10 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Immunohistochemistry - Anti-Smad3 (phospho Ser423 + Ser425) Antibody [ARC0170] (A308811)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Smad3 (phospho Ser423 + Ser425) Antibody [ARC0170] (A308811) at a dilution of 1:50(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Smad3 (phospho Ser423 + Ser425) Antibody [ARC0170] (A308811)

Immunohistochemistry analysis of paraffin-embedded mouse lung using Anti-Smad3 (phospho Ser423 + Ser425) Antibody [ARC0170] (A308811) at a dilution of 1:50(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Smad3 (phospho Ser423 + Ser425) Antibody [ARC0170] (A308811)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Smad3 (phospho Ser423 + Ser425) Antibody [ARC0170] (A308811) at a dilution of 1:50(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.