Anti-Smad3 (phospho Ser208) Antibody (A94166)

$190

Rabbit polyclonal antibody to Smad3 (phospho Ser208) for WB, IHC and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-Smad3 (phospho Ser208) Antibody

Description

Rabbit polyclonal antibody to Smad3 (phospho Ser208).

Specificity

This antibody detects endogenous levels of Smad3 only when phosphorylated at Ser208.

Applications

WB, IHC, ELISA

Dilutions

WB: 1:500-1:1000, ELISA: 1:5000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human Smad3 around the phosphorylation site of Ser208 (amino acids 174-223).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

48kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

hMAD-3, hSMAD3, JV15-2, MAD homolog 3, Mad3, MADH3, Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMAD 3, SMAD family member 3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

Western Blot - Anti-Smad3 (phospho Ser208) Antibody (A94166)

Western blot analysis of lysates from Jurkat cells using Anti-Smad3 (phospho Ser208) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

Immunohistochemistry - Anti-Smad3 (phospho Ser208) Antibody (A94166)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Anti-Smad3 (phospho Ser208) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

Western Blot - Anti-Smad3 (phospho Ser208) Antibody (A94166)

Western blot analysis of various cells using Anti-Smad3 (phospho Ser208) Antibody.

Western Blot - Anti-Smad3 (phospho Ser208) Antibody (A94166)

Western blot analysis of Jurkat cells using Anti-Smad3 (phospho Ser208) Antibody.

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