Anti-Smad3 (phospho Ser204) Antibody (A93814)

Name: Anti-Smad3 (phospho Ser204) Antibody
Brand: Antibodies.com
SKU: A93814
Price: 190.0 USD
Availability: InStock

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$190

Rabbit polyclonal antibody to Smad3 (phospho Ser204) for WB and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-Smad3 (phospho Ser204) Antibody

Description

Rabbit polyclonal antibody to Smad3 (phospho Ser204).

Specificity

This antibody detects endogenous levels of Smad3 only when phosphorylated at Ser204.

Applications

WB, ELISA

Dilutions

WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:10000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human Smad3 around the phosphorylation site of Ser204 (amino acids 170-219).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Concentration

1 mg/ml

Molecular Weight

48kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

hMAD-3, hSMAD3, JV15-2, MAD homolog 3, Mad3, MADH3, Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMAD 3, SMAD family member 3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-Smad3 (phospho Ser204) Antibody (A93814)

Western blot analysis of lysates from NIH/3T3 cells treated with Serum 20% 15' using Anti-Smad3 (phospho Ser204) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Western Blot - Anti-Smad3 (phospho Ser204) Antibody (A93814)

Western blot analysis of various cells using Anti-Smad3 (phospho Ser204) Antibody.

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Western Blot - Anti-Smad3 (phospho Ser204) Antibody (A93814)

Western blot analysis of MCF 7 cells using Anti-Smad3 (phospho Ser204) Antibody.

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Immunohistochemistry - Anti-Smad3 (phospho Ser204) Antibody (A93814)

Immunohistochemical analysis of paraffin-embedded human brain using Anti-Smad3 (phospho Ser204) Antibody 1:100 (4°C overnight). The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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