Anti-xCT Anticorpo (A84265)

Name: Anti-xCT Antibody
Brand: Antibodies.com
SKU: A84265
Price: 480.0 USD
Availability: InStock

Questo prodotto è identico a Abcam (ab111822). Scopri di più con meno.

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$480

Anticorpo policlonale di capra contro xCT per ELISA e IF.

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Garanzia di corrispondenza del prezzo

Consegna

Spese di spedizione

$40

Tempi di consegna

3-5 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

Garanzia di qualità al 100%

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Descrizione Prodotto

Nome

Anti-xCT Antibody

Descrizione

Goat polyclonal antibody to xCT.

Applicazioni

ELISA, IF

Diluizioni consigliate

ELISA: 1:32,000, IF: 10µg/ml

Reattività

Human

Immunogeno

Synthetic peptide corresponding to the internal region of human xCT.

Sequenza

C-KGQTQNFKDAFSGRD

Specie ospite

Goat

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentrazione

500 µg/ml

Forma del prodotto

Liquid

Formulazione

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Sinonimi

Amino acid transport system xc xCT, Amino acid transport system xc-, Calcium channel blocker resistance protein CCBR1, CCBR1, Cysteine/glutamate transporter, Cystine/glutamate transporter, OTTHUMP00000164578, SLC7A11, solute carrier family 7, Solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11, Solute carrier family 7 member 11, Solute carrier family 7, (cationic amino acid transporter, y+ system) member 11, SYSTEM Xc(-) TRANSPORTER-RELATED PROTEIN, XCT_HUMAN

Controlli isotipo

Goat IgG (A121671)

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (7)

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Figure 1: Immunofluorescence - Anti-xCT Antibody (A84265)

xCT expression in HepG2 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-xCT Antibody (A84265) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Vesicle staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 2: Immunofluorescence - Anti-xCT Antibody (A84265)

xCT expression in A549 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-xCT Antibody (A84265) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. ER/vesicle staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Western Blot - Anti-xCT Antibody (A84265)

xCT expression in Human Smooth Muscle (A) and Tonsil (B) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-xCT Antibody (A84265) at 0.1µg/ml and detected by chemiluminescence.

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Figure 4: Western Blot - Anti-xCT Antibody (A84265)

xCT expression in A549 (A) and U2OS (B) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-xCT Antibody (A84265) at 0.3µg/ml and detected by chemiluminescence.