Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Cyclin-A/CDK2-associated protein p19, EMC19, OCP-2, OCP-II, OCP2, Organ of Corti protein 2, Organ of Corti protein II, p19A, RNA polymerase II elongation factor-like protein, S-phase kinase-associated protein 1, SIII, SKP1A, TCEB1L
Western Blot - Anti-Skp1 Antibody [ARC1384] (A306186)
Western blot analysis of extracts of various cell lines, using Anti-Skp1 Antibody [ARC1384] (A306186) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunofluorescence analysis of NIH-3T3 cells using Anti-Skp1 Antibody [ARC1384] (A306186) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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