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Anti-SKIP Anticorpo (A15908)

Nome
Anti-SKIP Antibody
Descrizione
Rabbit polyclonal antibody to SKIP.
Applicazioni
WB, ICC/IF
Diluizioni consigliate
WB: 1:500-1:1,000, ICC/IF: 1:50-1:200
Reattività
Human, Mouse, Rat
Immunogeno
Recombinant fusion protein containing a sequence corresponding to amino acids 149-448 of human INPP5K (NP_057616.2).
Sequenza
HLPPHISNNYQRLEHFDRILEMQNCEGRDIPNILDHDLIIWFGDMNFRIEDFGLHFVRESIKNRCYGGLWEKDQLSIAKKHDPLLREFQEGRLLFPPTYKFDRNSNDYDTSEKKRKPAWTDRILWRLKRQPCAGPDTPIPPASHFSLSLRGYSSHMTYGISDHKPVSGTFDLELKPLVSAPLIVLMPEDLWTVENDMMVSYSSTSDFPSSPWDWIGLYKVGLRDVNDYVSYAWVGDSKVSCSDNLNQVYIDISNIPTTEDEFLLCYYSNSLRSVVGISRPFQIPPGSLREDPLGEAQPQI
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
51 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
43 kDa form skeletal muscle and kidney enriched inositol phosphatase, Inositol polyphosphate 5-phosphatase K, INP5K_HUMAN, INPP5K, muscle and kidney-enriched inositol phosphatase, PPS, skeletal muscle and kidney enriched inositol phosphatase, Skeletal muscle and kidney-enriched inositol phosphatase
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-SKIP Antibody (A15908) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-SKIP Antibody (A15908)
Western blot analysis of extracts of 293T cells, using Anti-SKIP Antibody (A15908) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western Blot - Anti-SKIP Antibody (A15908) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Western Blot - Anti-SKIP Antibody (A15908)
Western blot analysis of extracts of Mouse skeletal muscle, using Anti-SKIP Antibody (A15908) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Immunofluorescence - Anti-SKIP Antibody (A15908) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Immunofluorescence - Anti-SKIP Antibody (A15908)
Immunofluorescence analysis of A-549 cells using Anti-SKIP Antibody (A15908) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-SKIP Antibody (A15908) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Immunofluorescence - Anti-SKIP Antibody (A15908)
Immunofluorescence analysis of HeLa cells using Anti-SKIP Antibody (A15908) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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