Anti-Siglec 7 Anticorpo [S7.7] (A281727)

Anti-Siglec 7 Antibody [S7.7]

Mouse monoclonal [S7.7] antibody to Siglec 7.

This antibody recognises human Sialic acid-binding Ig-like lectin 7, otherwise known as CD328, a putative adhesion molecule and member of the Ig superfamily, predominantly expressed by both resting and activated natural killer (NK) cells, and also at lower levels by monocytes, granulocytes, monocyte-derived macrophages and dendritic cells, and a small population of CD8+ memory T cells. Classed as a CD33-related Siglec, Siglec-7 preferentially binds to glycoconjugates containing alpha-2,3- or alpha-2,6-linked sialic acid, and acts as a novel inhibitory receptor for NK cells, mediating the inhibition of NK cell cytotoxicity, and implicated in the regulation of NK cell and T cell activation, and hematopoiesis. Mouse anti Human Siglec-7 antibody, cloneS7.7 is a blocking antibody , and has been shown to recognize Siglec-7 in Rhesus monkey.

WB, IP, Flow Cytometry, ELISA

Flow Cytometry: 1:25 - 1:200, Use 10µl of the suggested working dilution to label 10⁶ cells.

Human, Rhesus Monkey

Siglec-7 3T3 transfected cells.

Mouse

Monoclonal

S7.7

IgG1

Unconjugated

Protein G affinity chromatography of tissue culture supernatant.

Approximately 65 kDa using Siglec-7 transfected CHO cells see Nicoll, G. et al. for details.

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human Siglec-7 antibody, cloneS7.7 recognizes human Sialic acid-binding Ig-like lectin 7, otherwise known as CD328, a putative adhesion molecule and member of the Ig superfamily, predominantly expressed by both resting and activated natural killer (NK) cells, and also at lower levels by monocytes, granulocytes, monocyte-derived macrophages and dendritic cells, and a small population of CD8+ memory T cells. Classed as a CD33-related Siglec, Siglec-7 preferentially binds to glycoconjugates containing alpha-2,3- or alpha-2,6-linked sialic acid, and acts as a novel inhibitory receptor for NK cells, mediating the inhibition of NK cell cytotoxicity, and implicated in the regulation of NK cell and T cell activation, and hematopoiesis. Mouse anti Human Siglec-7 antibody, cloneS7.7 is a blocking antibody (Avril et al. 2006), and has been shown to recognize Siglec-7 in Rhesus monkey (Jaroenpool et al. 2007).

Adhesion inhibitory receptor molecule 1, Adhesion inhibitory receptor molecule 1, siglec 7, AIRM 1, AIRM-1, AIRM1, CD328, CD328 antigen, CDw328, D siglec, D-siglec, HLDA 8, p75, p75/AIRM1, QA79, QA79 membrane protein, Sialic acid binding Ig like lectin 7, Sialic acid binding immunoglobulin like lectin 7, Sialic acid-binding Ig-like lectin 7, SIGL7_HUMAN, Siglec-7, SIGLEC19P, SIGLEC7, SIGLECP2