Si-DMA for Mitochondrial Singlet Oxygen Imaging (A57287)

Majima et al. synthesized a new far-red fluorescence probe composed of silicon-containing rhodamine and anthracene moieties, namely Si-DMA, as a chromophore and a 1O2 reactive site, respectively. In the presence of 1O2, fluorescence of Si-DMA increases 17 times due to endoperoxide formation at the anthracene moiety. Among seven different ROS, Si-DMA is able to selectively detect the 1O2. In addition, Si-DMA is able to visualize the real-time generation of 1O2 from protoporphyrin IX in mitochondria with 5-aminolevulinic acid (5-ALA), a precursor of heme.