Anti-SHP2 (phospho Tyr542) Anticorpo (A16556)

Name: Anti-SHP2 (phospho Tyr542) Antibody
Brand: Antibodies.com
SKU: A16556
Price: 435.0 USD
Availability: InStock

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$435

Anticorpo policlonale di coniglio contro SHP2 (phospho Tyr542) per WB e IHC.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-SHP2 (phospho Tyr542) Antibody

Descrizione

Rabbit polyclonal antibody to SHP2 (phospho Tyr542).

Applicazioni

WB, IHC

Diluizioni consigliate

WB: 1:500-1:1,000, IHC: 1:50-1:100

Reattività

Human, Mouse, Rat

Immunogeno

A synthetic phosphorylated peptide around Y542 of human PTPN11 (NP_002825.3).

Sequenza

HEYTN

Specie ospite

Rabbit

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

72 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

BPTP3, CFC, JMML, METCDS, MGC14433, NS1, OTTHUMP00000166107, OTTHUMP00000166108, Protein tyrosine phosphatase 2, Protein tyrosine phosphatase 2C, Protein tyrosine phosphatase non receptor type 11, Protein-tyrosine phosphatase 1D, Protein-tyrosine phosphatase 2C, PTN11_HUMAN, PTP-1D, PTP-2C, PTP1D, PTP2C, PTPN11, SAP2, SH-PTP2, SH-PTP3, SH2 domain containing protein tyrosine phosphatase 2, SHP 2, SHP-2, SHPTP2, SHPTP3, Syp, Tyrosine-protein phosphatase non-receptor type 11

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (3)

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Figure 1: Western Blot - Anti-SHP2 (phospho Tyr542) Antibody (A16556)

Western blot analysis of NIH/3T3, using Anti-SHP2 (phospho Tyr542) Antibody (A16556) at 1:700 dilution. NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Western Blot - Anti-SHP2 (phospho Tyr542) Antibody (A16556)

Western blot analysis of C6, using Anti-SHP2 (phospho Tyr542) Antibody (A16556) at 1:700 dilution. C6cells were treated by PDGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Immunohistochemistry - Anti-SHP2 (phospho Tyr542) Antibody (A16556)

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-SHP2 (phospho Tyr542) Antibody (A16556). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.