Anti-SHIP-1 Antibody (A8360)

Name: Anti-SHIP-1 Antibody
Brand: Antibodies.com
SKU: A8360
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to SHIP-1 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-SHIP-1 Antibody

Description

Rabbit polyclonal antibody to SHIP-1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 900-1000 of human SHIP1 (NP_001017915.1).

Sequence

PPCSGSSITEIINPNYMGVGPFGPPMPLHVKQTLSPDQQPTAWSYDQPPKDSPLGPCRGESPPTPPGQPPISPKKFLPSTANRGLPPRTQESRPSDLGKNA

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

140 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

EC:3.1.3.36, EC:3.1.3.56, hp51CN, Inositol polyphosphate-5-phosphatase D, Inositol polyphosphate-5-phosphatase of 145 kDa, INPP5D, p150Ship, Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1, Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, SH2 domain-containing inositol 5'-phosphatase 1, SH2 domain-containing inositol phosphatase 1, SHIP, SIP-145

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-SHIP-1 Antibody (A8360)

Western blot analysis of extracts of Mouse spleen, using Anti-SHIP-1 Antibody (A8360) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Immunohistochemistry - Anti-SHIP-1 Antibody (A8360)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-SHIP-1 Antibody (A8360) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-SHIP-1 Antibody (A8360)

Immunofluorescence analysis of mouse spleen cells using Anti-SHIP-1 Antibody (A8360) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-SHIP-1 Antibody (A8360)

Immunofluorescence analysis of THP-1 cells using Anti-SHIP-1 Antibody (A8360) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).

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