Anti-Shigella Aptamer [Sp1] (Biotin) (A321127)

Spese di spedizione
Tempi di consegna
30-45 Giorni lavorativi
Telefono
+1 (314) 370-6046
Lun - Ven, 8:00 - 16:00 AST
E-mail
orders@antibodies.com
Name
Anti-Shigella Aptamer [Sp1] (Biotin)
Description
Synthetic DNA aptamer to Shigella [Sp1] (Biotin).
Specificity
Binding with Shigella sonnei is six times greater than other, related bacteria species.
Conjugate

Biotin

Aptamer Resuspension
Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.
Apatmer Binding Conditions
Binding Buffer: 50mM Tris-HCl Buffer, pH 7.4, with 5mM Potassium Chloride, 100mM Sodium Chloride, and 1mM Magnesium Chloride. Binding Temperature: 37°C.
Sequence
'5-dTpdGpdApdGpdCpdCpdCpdApdApdGpdCpdCpdCpdTpdGpdGpdTpdApdTpdGpdTpdTpdCpdTpdTpdCpdCpdCpdTpdTpdTpdTpdApdTpdTpdApdGpdTpdCpdCpdTpdGpdTpdApdTpdTpdCpdCpdTpdCpdTpdApdCpdTpdGpdTpdTpdGpdCpdCpdGpdGpdCpdApdGpdGpdTpdCpdTpdApdCpdTpdTpdTpdGpdGpdGpdApdTpdCp -'3
Aptamer Length
80 nt
Aptamer Molecular Weight (Da)
24,547.59 Da
Aptamer Affinity
Affinity (Kd) = 5.98 nM
Extinction Coefficient
720600 M-1cm-1
Purification
HPLC
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nuclease-free water (no residual salts).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.
General Notes
Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 30-45 business days.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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