Rabbit polyclonal antibody to Shc1 (Phospho-Tyr427)
Specificity
The antibody detects endogenous level of Shc1 only when phosphorylated at tyrosine 427.
Applications
WB, IHC, IF
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of tyrosine 427 (P-S-Y(p)-V-N derived from Human Shc1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Synonyms
SH2 domain protein C1, SHC-transforming protein 1, SHC-transforming protein 3, SHC-transforming protein A, SHCA, Src homology 2 domain-containing-transforming protein C1
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Shc1 (Phospho-Tyr427) Antibody #11317 (left) or the same antibody preincubated with blocking peptide (right).
