Rabbit polyclonal antibody to Shc (phospho Tyr427).
Specificity
This antibody detects endogenous levels of Shc only when phosphorylated at Tyr427.
Applications
WB, IHC, ELISA
Dilutions
WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:10000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide derived from human Shc around the phosphorylation site of Tyr427 (amino acids 393-442).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
62kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
SH2 domain protein C1, SHC-transforming protein 1, SHC-transforming protein 3, SHC-transforming protein A, SHC1, SHCA, Src homology 2 domain-containing-transforming protein C1
Western Blot - Anti-Shc (phospho Tyr427) Antibody (A94644)
Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 5' using Anti-Shc (phospho Tyr427) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunohistochemical analysis of paraffin-embedded human brain using Anti-Shc (phospho Tyr427) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Immunohistochemical analysis of paraffin-embedded human colon cancer using Anti-Shc (phospho Tyr427) Antibody 1:100 (4°C overnight). The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
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