SH3BP1 expression in Human Brain lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-SH3BP1 Antibody (A84005) at 1µg/ml and detected by chemiluminescence.
SH3BP1 expression in Human Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by microwaving in citrate buffer, pH 6. Staining was performed with Anti-SH3BP1 Antibody (A84005) at 0.3µg/ml and revealed with horseradish peroxidase (HRP).
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