Figure 1: Western Blot - Anti-SEMA4F Antibody (A12936)
Western blot analysis of extracts of various cell lines, using Anti-SEMA4F Antibody (A12936) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-SEMA4F Antibody (A12936) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Prodotti alternativi a Anti-SEMA4F Anticorpo (A12936)