Figure 1: Western Blot - Anti-SARS-CoV-2 Nucleoprotein Antibody (A307731)
Western blot analysis of extracts of normal 293T cells and 293T transfected with Nucleoprotein, using Anti-SARS-CoV-2 Nucleoprotein Antibody (A307731) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Figure 2: Western Blot - Anti-SARS-CoV-2 Nucleoprotein Antibody (A307731)
Western blot analysis of Recombinant Nucleoprotein of Coronavirus SARS-CoV-2，using Anti-SARS-CoV-2 Nucleoprotein Antibody (A307731) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Anti-SARS-CoV-2 Nucleoprotein Antibody (A307731) can bind immobilized recombinant SARS-COV-2 Nucleocapsid protein at 1 µg/ml (100 µl/well) with a linear range of 3.12-200ng/ml.
Immunofluorescence analysis of SARS-CoV-2 Nucleoprotein transfect Hela cells using Anti-SARS-CoV-2 Nucleoprotein Antibody (A307731). DAPI was used to stain the cell nuclei (blue).
Figure 5: Western Blot - Anti-SARS-CoV-2 Nucleoprotein Antibody (A307731)
Immunoprecipitation analysis of 300µg extracts of 293T cells using 3µg of Anti-SARS-CoV-2 Nucleoprotein Antibody (A307731). This Western blot was performed on the immunoprecipitate using Anti-SARS-CoV-2 Nucleoprotein Antibody (A307731) at a dilution of 1:1000.
Figure 6: Western Blot - Anti-SARS-CoV-2 Nucleoprotein Antibody (A307731)
Immunoprecipitation analysis of 300µg extracts of 293T cells using 3µg of Anti-SARS-CoV-2 Nucleoprotein Antibody (A307731). This Western blot was performed on the immunoprecipitate using Anti-SARS-CoV-2 Nucleoprotein Antibody (A307731) at a dilution of 1:3000.
