Anti-S100A9 Anticorpo (A84636)

Name: Anti-S100A9 Antibody
Brand: Antibodies.com
SKU: A84636
Price: 480.0 USD
Availability: InStock

Questo prodotto è identico a Novus (NBP1-18843). Scopri di più con meno.

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$480

Anticorpo policlonale di capra contro S100A9 per ELISA, WB, IHC, IF e Flow Cytometry.

Garanzia al 100%

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Consegna

Spese di spedizione

$40

Tempi di consegna

3-5 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

Garanzia di qualità al 100%

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Descrizione Prodotto

Nome

Anti-S100A9 Antibody

Descrizione

Goat polyclonal antibody to S100A9.

Applicazioni

ELISA, WB, IHC, IF, Flow Cytometry

Diluizioni consigliate

ELISA: 1:8,000, WB: 0.5-2µg/ml, IHC: 2.5ug/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reattività

Human

Immunogeno

Synthetic peptide corresponding to the internal region of human S100A9.

Sequenza

C-DTNADKQLSFEEF

Specie ospite

Goat

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentrazione

500 µg/ml

Peso molecolare

13-14 kDa

MW previsto

13.2kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Sinonimi

60B8AG, CAGB, Calgranulin B, Calgranulin-B, Calprotectin L1H subunit, CFAG, CGLB, Cystic fibrosis antigen B, L1AG, Leukocyte L1 complex heavy chain, LIAG, MAC387, MIF, Migration inhibitory factor related protein 14, Migration inhibitory factor-related protein 14, MRP 14, MRP-14, MRP14, Myeloid-related protein 14, NIF, OTTHUMP00000015331, p14, Protein S100-A9, S100 A9, S100 calcium binding protein A9, S100 calcium binding protein A9 calgranulin B, S100 calcium-binding protein A9, S10A9_HUMAN

Controlli isotipo

Goat IgG (A121671)

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (7)

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Figure 1: Western Blot - Anti-S100A9 Antibody (A84636)

S100A9 expression in Human Bone Marrow (A), Gastrointestinal cancer (B), and negative control HepG2 (C) lysate analyzed by western blot. Cells were lysed in RIPA buffer and 5µg protein was run per lane. Primary antibody was performed with Anti-S100A9 Antibody (A84636) at 1µg/ml (A) and 0.5µg/ml (B) and detected by chemiluminescence.

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Figure 2: Immunofluorescence - Anti-S100A9 Antibody (A84636)

S100A9 expression in MCF7 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-S100A9 Antibody (A84636) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Immunofluorescence - Anti-S100A9 Antibody (A84636)

S100A9 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-S100A9 Antibody (A84636) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 4: Flow Cytometry - Anti-S100A9 Antibody (A84636)

S100A9 expression in MCF7 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-S100A9 Antibody (A84636) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.