Figure 1: Western Blot - Anti-S100 alpha 6 / PRA Antibody (A11719)
Western blot analysis of extracts from normal (control) and S100A6 knockout (KO) HeLa cells, using Anti-S100 alpha 6 / PRA Antibody (A11719) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Figure 2: Western Blot - Anti-S100 alpha 6 / PRA Antibody (A11719)
Western blot analysis of extracts of various cell lines, using Anti-S100 alpha 6 / PRA Antibody (A11719) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
