Recombinant rabbit monoclonal [RRM1/4372R] antibody to RRM1.
Specificity
Ribonucleotide reductase M1 polypeptide (RRM1) is one of two non-identical subunits for ribonucleoside-diphosphate reductase, an enzyme which catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. It provides the precursors necessary for DNA synthesis. RRM1 is present throughout the cell division cycle but downregulated in quiescent cells. RRM1 is involved in carcinogenesis, tumor progression, and the response of non-small-cell lung cancer (NSCLC) to chemotherapy.
Applications
IHC-P
Dilutions
IHC-P: 1-2 µg/ml
Reactivity
Human
Immunogen
Synthetic peptide, around amino acids 700-800, of human RRM1 protein. The exact sequence is proprietary.
Host
Rabbit
Clonality
Monoclonal
Clone ID
RRM1/4372R
Isotype
IgG
Conjugate
Unconjugated
Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
94 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Negative Tissue Control: Immunohistochemical analysis of formalin-fixed, paraffin-embedded human brain using Anti-RRM1 Antibody [RRM1/4372R] at 2µg/ml in PBS for 30 minutes at room temperature.
SDS-PAGE analysis of Anti-RRM1 Antibody [RRM1/4372R] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
Analysis of protein array containing more than 19,000 full-length human proteins using Anti-RRM1 Antibody [RRM1/4372R]. Z-Score and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target; a MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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