Anti-ROR gamma Anticorpo (A82850)

Name: Anti-ROR gamma Antibody
Brand: Antibodies.com
SKU: A82850
Price: 480.0 USD
Availability: InStock

Questo prodotto è identico a Abcam (ab113434). Scopri di più con meno.

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$480

Anticorpo policlonale di capra contro ROR gamma per ELISA, WB, IF e Flow Cytometry.

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Consegna

Spese di spedizione

$40

Tempi di consegna

3-5 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

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Descrizione Prodotto

Nome

Anti-ROR gamma Antibody

Descrizione

Goat polyclonal antibody to ROR gamma.

Applicazioni

ELISA, WB, IF, Flow Cytometry

Diluizioni consigliate

ELISA: 1:16,000, WB: 0.01-0.1µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reattività

Human

Immunogeno

Synthetic peptide corresponding to the internal region, amino acids 200-212, of human ROR gamma.

Sequenza

CHLEYSPERGKAE

Specie ospite

Goat

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentrazione

500 µg/ml

Peso molecolare

55-60 kDa

MW previsto

55.8kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Sinonimi

IMD42, MGC129539, NR1F3, Nuclear receptor ROR gamma, Nuclear receptor ROR-gamma, Nuclear receptor RZR gamma, Nuclear receptor RZR-gamma, Nuclear receptor subfamily 1 group F member 3, RAR related orphan nuclear receptor variant 2, RAR related orphan receptor C, RAR related orphan receptor C, isoform a, RAR related orphan receptor gamma, RAR-related orphan receptor C, Retinoic acid binding receptor gamma, Retinoid related orphan receptor gamma, Retinoid-related orphan receptor-gamma, Rorc, RORG, RORG_HUMAN, RZR GAMMA, RZRG, TOR

Controlli isotipo

Goat IgG (A121671)

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (3)

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Figure 1: Western Blot - Anti-ROR gamma Antibody (A82850)

ROR gamma expression in Human Colon (A), Liver (B), and Testes (C) lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-ROR gamma Antibody (A82850) at 0.1µg/ml (A), 0.03µg/ml (B), or 0.01µg/ml (C) and detected by chemiluminescence.

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Figure 2: Immunofluorescence - Anti-ROR gamma Antibody (A82850)

ROR gamma expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-ROR gamma Antibody (A82850) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic, plasma membrane, and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Flow Cytometry - Anti-ROR gamma Antibody (A82850)

ROR gamma expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-ROR gamma Antibody (A82850) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.