Anti-ROCK1 Anticorpo (A12783)

Name: Anti-ROCK1 Antibody
Brand: Antibodies.com
SKU: A12783
Price: 470.0 USD
Availability: InStock

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$470

Anticorpo policlonale di coniglio contro ROCK1 per WB e IHC.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Tag dei prodotti:

Anticorpo Knockout Convalidato

Descrizione Prodotto

Nome

Anti-ROCK1 Antibody

Descrizione

Rabbit polyclonal antibody to ROCK1.

Applicazioni

WB, IHC

Diluizioni consigliate

WB: 1:500-1:1,000, IHC: 1:50-1:100

Reattività

Human, Mouse, Rat

Immunogeno

Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human ROCK1 (NP_005397.1).

Sequenza

YSNRRYLSSANPNDNRTSSNADKSLQESLQKTIYKLEEQLHNEMQLKDEMEQKCRTSNIKLDKIMKELDEEGNQRRNLESTVSQIEKEKMLLQHRINEYQRKAEQENEKRRNVENEVSTLKDQLEDLKKVSQNSQLANEKLSQLQKQLEEANDLLRTESDTAVRLRKSHTEMSKSISQLESLNRELQERNRILENSKSQTDKDYYQLQAILEAERRDRGHDSEMIGDLQARITSLQEEVKHLKHNLEKVEGERKEAQDMLN

Specie ospite

Rabbit

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

158 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

coiled-coil-containing protein kinase 1, coiled-coil-containing protein kinase I, MGC131603, MGC43611, p160 Rhoassociated coiled coil-forming protein kinase, p160 ROCK-1, p160 ROCK1, p160ROCK, PRO0435, Renal carcinoma antigen NY REN 35, Renal carcinoma antigen NY-REN-35, Rho associated coiled coil containing protein kinase 1, Rho associated protein kinase 1, Rho kinase, Rho-alpha kinase, Rho-associated, Rho-associated protein kinase 1, ROCK I, ROCK-I, ROCK1_HUMAN, Rok, rokalpha

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (4)

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Figure 1: Western Blot - Anti-ROCK1 Antibody (A12783)

Western blot analysis of various lysates, using Anti-ROCK1 Antibody (A12783) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-ROCK1 Antibody (A12783)

Western blot analysis of C6, using Anti-ROCK1 Antibody (A12783) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Western Blot - Anti-ROCK1 Antibody (A12783)

Western blot analysis of extracts from wild type(WT) and ROCK1 knockout (KO) 293T cells, using Anti-ROCK1 Antibody (A12783) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 4: Immunohistochemistry - Anti-ROCK1 Antibody (A12783)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-ROCK1 Antibody (A12783) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.