Recombinant Human IL-2 Protein (mutated L100F + R101D + L105V + I106V + I112F) (6xHis Tag)
Applicazioni
Cell Proferation Assay, SDS-PAGE
Sistema di espressione
HEK293 cells
Tipo di proteina
Recombinant
Specie proteiche
Human
Lunghezza
Protein fragment.
Aminoacidi
Ala21-Thr153 (L100F, R101D, L105V, I106V, I112F)
Etichetta
C-terminal 6xHis Tag
Peso molecolare
16.5 KDa
Coniugare
Unconjugated
Purezza
> 95%, by SDS-PAGE under reducing conditions.
Forma del prodotto
Lyophilized
Concentrazione
Reconstitution dependent.
Formulazione
Lyophilized from a 0.2 µm filtered solution of 4mM HCl.
Conservazione
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Sinonimi
Aldesleukin, IL 2, IL2, IL2_HUMAN, Interleukin 2, Interleukin-2, Interleukin2, Involved in regulation of T cell clonal expansion, Lymphokine , OTTHUMP00000164090, POIL2, T cell growth factor, T-cell growth factor, TCGF
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Figure 2: Recombinant Human IL-2 Protein (mutated L100F + R101D + L105V + I106V + I112F) (6xHis Tag) (A317605)
Loaded Human IL-2RB-Fc on Protein A Biosensor, can bind Recombinant Human IL-2 Protein (mutated L100F + R101D + L105V + I106V + I112F) (6×His Tag) (A317605) with an affinity constant of 0.58 nM as determined in BLI assay.
Figure 3: Recombinant Human IL-2 Protein (mutated L100F + R101D + L105V + I106V + I112F) (6xHis Tag) (A317605)
Measurement of Recombinant Human IL-2 Protein (mutated L100F + R101D + L105V + I106V + I112F) (6×His Tag) (A317605) in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED50 for this effect is 151.39 pg/ml.
Figure 4: Recombinant Human IL-2 Protein (mutated L100F + R101D + L105V + I106V + I112F) (6xHis Tag) (A317605)
Loaded Human IL-2RA-Fc on Protein A Biosensor, can bind Recombinant Human IL-2 Protein (mutated L100F + R101D + L105V + I106V + I112F) (6×His Tag) (A317605) with an affinity constant of 10.4 nM as determined in BLI assay.
