0.7 Units. The enzymatic activity of each Heparanase Active Enzyme batch is comparable to the standard as determined by activity assay in which immobilized heparan, released due to heparanase activity, is quantified colorimetrically. Recommended reaction buffer: 20 mM Citrate Phosphate buffer, pH 5.4; 50mM NaCl; 1mM CaCl2.
Definizione dell'unità
One unit is defined as 1 µmole of reducing ends of heparan sulfate substrate formed per minute per mg Heparanase Active Enzyme at 37°C
Purezza
>95% as determined by RP-HPLC and SDS-PAGE.
Forma del prodotto
Liquid
Concentrazione
Lot Specific
Formulazione
Supplied in 20 mM Acetate Buffer, pH 5.4, with 750 mM Sodium Chloride.
Conservazione
Shipped on dry ice. Store at -80°C. Avoid freeze/thaw cycles.