Recombinant Human CD276 Protein (Fc Chimera 6xHis Tag)
Applicazioni
ELISA, SDS-PAGE
Sistema di espressione
HEK293 cells
Tipo di proteina
Recombinant
Specie proteiche
Human
Lunghezza
Protein fragment.
Sequenza
B7-H3 (Leu29-Pro245) + mFc (Pro99-Lys330) + 6xHis Tag
Etichetta
C-terminal Mouse Fc Tag and 6xHis Tag
Peso molecolare
The protein has a predicted molecular mass of 70-75 kDa after removal of the signal peptide.
Coniugare
Unconjugated
Purezza
> 95%, by SDS-PAGE and Coomassie blue staining.
Forma del prodotto
Lyophilized
Concentrazione
Reconstitution dependent.
Formulazione
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Conservazione
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Figure 2: Recombinant Human CD276 Protein (Fc Chimera 6xHis Tag) (A318396)
ELISA plates were pre-coated with Recombinant Human CD276 Protein (Fc Chimera 6xHis Tag) (A318396) at 2 µg/ml (100 µl/well) which can bind Anti-B7-H3 Neutralizing antibody in a linear range of 0.24-31.25 ng/ml.
Figure 3: Recombinant Human CD276 Protein (Fc Chimera 6xHis Tag) (A318396)
ELISA plates were pre-coated with Recombinant Human CD276 Protein (Fc Chimera 6xHis Tag) (A318396) at 2 µg/ml (100 µl/well) which can bind TAA06 Biosimilar - Anti-CD276 Antibody - BSA and Azide free (A324692) in a linear range of 0.64-16 ng/ml.
