>70 U/mg obtained by measuring the increase of beta-NADPH in absorbance at 340 nm resulting from the reduction beta- NADP.
Definizione dell'unità
One unit is defined as the ammount of enzyme that will oxidize 1 µmole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25°C.
Purezza
>90% as determined by SDS-PAGE.
Forma del prodotto
Liquid
Concentrazione
Lot Specific
Formulazione
Supplied in 50 mM MES Buffer, pH 6, with 100 µM PMSF, 2 mM EDTA, 500 µM DTT, and 10% Glycerol.
Conservazione
Shipped at 4°C. Short term (2-4 weeks) store at 4°C. Long term store at -20°C, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze/thaw cycles.