Recombinant E. coli Glucokinase Protein (N-terminal His Tag) (A325813)

Overview
Specifications

Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Tags:

Biologically Active

Specifications

Name

Recombinant E. coli Glucokinase Protein (N-terminal His Tag)

Applications

SDS-PAGE

Expression System

Escherichia coli

Nature

Recombinant

Protein Species

E. coli

Protein Length

Full length protein

Sequence

MGSSHHHHHH SSGLVPRGSH MGSMTKYALV GDVGGTNARL ALCDIASGEI SQAKTYSGLD YPSLEAVIRV YLEEHKVEVK DGCIAIACPI TGDWVAMTNH TWAFSIAEMK KNLGFSHLEI INDFTAVSMA IPMLKKEHLI QFGGAEPVEG KPIAVYGAGT GLGVAHLVHV DKRWVSLPGE GGHVDFAPNS EEEAIILEIL RAEIGHVSAE RVLSGPGLVN LYRAIVKADN RLPENLKPKD ITERALADSC TDCRRALSLF CVIMGRFGGN LALNLGTFGG VFIAGGIVPR FLEFFKASGF RAAFEDKGRF KEYVHDIPVY LIVHDNPGLL GSGAHLRQTL GHIL

Tag

N-terminal His Tag

Predicted MW

37.1 kDa

Biologically Active

Yes

Biological Activity

>70 U/mg obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP.

Unit Definition

One unit is defined as the ammount of enzyme that will oxidize 1 µmole of Glucose to D-glucose 6-phosphate per minute in the presence of Beta-NADP at pH 9.0 at 37C.

Purity

>95% as determined by SDS-PAGE.

Product Form

Liquid

Concentration

1 mg/ml

Formulation

Supplied in 20 mM Tris HCl Buffer, pH 8, with 150 mM Sodium Chloride and 10% Glycerol.

Storage

Shipped at 4°C. Short term (2-4 weeks) store at 4°C. Long term store at -20°C, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze/thaw cycles.

Synonyms

GCK, Hexokinase type IV, Hexokinase-4, Hexokinase-D, HK IV, HK4

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

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