Anti-RBPJK Antibody [ARC0894] (A305486)

Name: Anti-RBPJK Antibody [ARC0894]
Brand: Antibodies.com
SKU: A305486
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC0894] antibody to RBPJK for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-RBPJK Antibody [ARC0894]

Description

Rabbit monoclonal [ARC0894] antibody to RBPJK.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human RBPJK (Q06330).

Sequence

CAFIGIGNSDQEMQQLNLEGKNYCTAKTLYISDSDKRKHFMLSVKMFYGNSDDIGVFLSKRIKVISKPSKKKQSLKNADLCIASGTKVALFNRLRSQTVST

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0894

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

67 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

CBF-1, IGKJRB, IGKJRB1, J kappa-recombination signal-binding protein, RBP-J, RBP-J kappa, RBP-JK, RBPSUH, Recombining binding protein suppressor of hairless, Renal carcinoma antigen NY-REN-30

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-RBPJK Antibody [ARC0894] (A305486)

Western blot analysis of extracts of various cell lines, using Anti-RBPJK Antibody [ARC0894] (A305486) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Immunohistochemistry - Anti-RBPJK Antibody [ARC0894] (A305486)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-RBPJK Antibody [ARC0894] (A305486) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-RBPJK Antibody [ARC0894] (A305486)

Immunohistochemistry analysis of paraffin-embedded human lung squamous carcinoma tissue using Anti-RBPJK Antibody [ARC0894] (A305486) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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