Anti-Rb (phospho Ser795) Anticorpo (A16439)

Name: Anti-Rb (phospho Ser795) Antibody
Brand: Antibodies.com
SKU: A16439
Price: 435.0 USD
Availability: InStock

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$435

Anticorpo policlonale di coniglio contro Rb (phospho Ser795) per WB e IHC.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-Rb (phospho Ser795) Antibody

Descrizione

Rabbit polyclonal antibody to Rb (phospho Ser795).

Applicazioni

WB, IHC

Diluizioni consigliate

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reattività

Human, Mouse, Rat

Immunogeno

A synthetic phosphorylated peptide around S795 of human RB (NP_000312.2).

Sequenza

PSSPL

Specie ospite

Rabbit

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

106 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

Exon 17 tumor GOS561 substitution mutation causes premature stop, GOS563 exon 17 substitution mutation causes premature stop, OSRC, Osteosarcoma, p105-Rb, P105RB, PP105, pp110, PPP1R130, pRb, Prepro retinoblastoma associated protein, Protein phosphatase 1 regulatory subunit 130, RB transcriptional corepressor 1, RB1, RB1 gene, RB_HUMAN, Retinoblastoma 1, Retinoblastoma suspectibility protein, Retinoblastoma-associated protein

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (5)

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Figure 1: Western Blot - Anti-Rb (phospho Ser795) Antibody (A16439)

Western blot analysis of extracts of 293 cell line, using Anti-Rb (phospho Ser795) Antibody (A16439). The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA.

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Figure 2: Western Blot - Anti-Rb (phospho Ser795) Antibody (A16439)

Western blot analysis of extracts of K-562 cells, using Anti-Rb (phospho Ser795) Antibody (A16439) at 1:1,000 dilution or RB1 antibody (A16966). K-562 cells were treated by ATP(5 mM) at 30°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 3: Immunohistochemistry - Anti-Rb (phospho Ser795) Antibody (A16439)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-Rb (phospho Ser795) Antibody (A16439) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Rb (phospho Ser795) Antibody (A16439)

Immunohistochemistry analysis of paraffin-embedded mouse lung using Anti-Rb (phospho Ser795) Antibody (A16439) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.