Rat TGF-β1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of rat TGF-β1 in the samples. A monoclonal antibody specific for rat TGF-β1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, and TGF-β1 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked monoclonal antibody specific for TGF-β1 is added to the wells. Following a wash to remove any unbound detect antibody, streptavidin-HRP is added. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of TGF-β1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Piattaforma

Microplate

Tipo di rilevamento

Colorimetric

Tipo di campione

Cell culture supernatant, serum and plasma.

Sensibilità

3.36 pg/ml

Campo di rilevamento

31.25 - 2000 pg/ml

Recupero

99% (Average Recovery from Serum Samples).

Specificità

This kit can assay both natural and recombinant rat TGF-β1.

Reattività

Rat

Reattività incrociata

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Conservazione

Store at 2 - 8°C.

Sinonimi

Camurati-Engelmann disease, Cartilage-inducing factor, CED, Differentiation inhibiting factor, DPD1, LAP, Latency-associated peptide, Prepro transforming growth factor beta 1, TGF beta, TGF beta 1 protein , TGF-beta 1 protein, TGF-beta-1, TGF-beta-5, TGF-beta1, TGFB, Tgfb-1, tgfb1, TGFB1_HUMAN, TGFbeta, TGFbeta1, Transforming Growth Factor b1, Transforming growth factor beta 1, Transforming growth factor beta 1a, transforming growth factor beta-1, transforming growth factor, beta 1, Transforming Growth Factor-ß1

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.