Ratto Laminin Kit Elisa (A641)

$615
Spese di spedizione
Tempi di consegna
7-10 Giorni lavorativi
Telefono
+1 (314) 370-6046
Lun - Ven, 8:00 - 16:00 AST
E-mail
orders@antibodies.com
Nome
Rat Laminin ELISA Kit
Tipo di kit
Sandwich (quantitative)
Principio di kit
This Laminin enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-Laminin Antibody. Standards or samples are then added to the microtire plate wells and Laminin, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of Laminin present in the sample, Anti-Laminin Antibody (Biotin) is added to each well to "sandwich" the Laminin immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Laminin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.
Piattaforma
Microplate
Tipo di rilevamento
Colorimetric
Tipo di campione
Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.
Sensibilità
< 10 pg/ml
Campo di rilevamento
156-10,000 pg/ml
Reattività
Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Conservazione
Store at 2-8°C for 4 months or at -20°C for 8 months.
Sinonimi
LAMA, LAMA 1, LAMA1, LAMB1, Lamb2, LAMB2_HUMAN, LAMC1, LAMC2, Lamc3, LAMC3_HUMAN, laminin A, Laminin A chain, Laminin alpha 1, Laminin alpha 1 chain, Laminin B, Laminin B1 chain, Laminin B1s chain, Laminin gamma 1, Laminin gamma 2, Laminin gamma 3, Laminin subunit beta 1, Laminin subunit beta-2, Laminin subunit gamma-3, Laminin-11 subunit beta, Laminin-12 subunit gamma, Laminin-14 subunit beta, Laminin-14 subunit gamma, Laminin-15 subunit beta, Laminin-15 subunit gamma, Laminin-3 subunit beta, Laminin-4 subunit beta, Laminin-7 subunit beta, Laminin-9 subunit beta, OCCM, S-LAM beta, S-laminin subunit beta
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

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