Rat sICAM-1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of rat sICAM-1 in the samples. A monoclonal antibody specific for rat sICAM-1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for sICAM-1, and sICAM-1 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of sICAM-1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Piattaforma

Microplate

Tipo di rilevamento

Colorimetric

Tipo di campione

Cell culture supernatant, serum and plasma.

Sensibilità

0.48 pg/ml

Campo di rilevamento

62.50 - 4000 pg/ml

Recupero

83% (Average Recovery from Serum Samples).

Specificità

This kit can assay both natural and recombinant rat sICAM-1.

Reattività

Rat

Reattività incrociata

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Conservazione

Store at 2 - 8°C.

Sinonimi

Antigen identified by monoclonal, BB 2, BB2, CD 54 , CD54, CD54 antigen , CD_antigen=CD54, Cell surface glycoprotein P3.58 , Human rhinovirus receptor , ICAM 1, ICAM-1, ICAM1_HUMAN, Intercellular adhesion molecule 1 , intercellular adhesion molecule 1 (CD54), human rhinovirus receptor, Major group rhinovirus receptor , MALA 2, MALA2 , MGC6195, MyD 10, MyD10, P3.58 , Surface antigen of activated B cells , Surface antigen of activated B cells, BB2

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.