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Anti-Rag A Anticorpo (A398)

Questo prodotto è identico a Abcam (ab128196). Scopri di più con meno.

Western blot analysis of RagA protein in the whole cell extracts (HeLa cell g). Anti-RagA antiserum was used at 1,000 dilution. Western blot analysis of RagA protein in the whole cell extracts. 1. HeLa (humans) 2. MCF7 (human) 3. MOLT4 (human) 4. NIH3T3 (mouse) Proteins in the extracts (20 µg) were separated on 12.5% SDS-PAGE. Anti-RagA antibody was used at 1/1,000 dilution?Anti-rabbit IgG antibody conjugated with HRP (Abcam;ab97051) was used as the 2nd antibody at 1/10,000 dilution Immunofluorescence staining of RagA protein in HeLa cells with anti-Rag antibody. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.5% TritonX 100 and reacted with anti-RagA antibody at 1/100 dilution (A) or at 1/1,000 dilution (B). As the 2nd antibody, anti-rabbit IgG antibody conjugated with Alexa Fluor 647 was used at 1/1,000 dilution. DNA was stained with DAPI and the merged image was shown on right
$510

Anticorpo policlonale di coniglio contro Rag A per WB.

Garanzia al 100%
Garanzia di corrispondenza del prezzo

Spese di spedizione
$40
Tempi di consegna
4-6 Giorni lavorativi

Telefono
+1 (314) 370-6046
Lun - Ven, 8:00 - 16:00 AST
E-mail
orders@antibodies.com

Nome
Anti-Rag A Antibody
Descrizione
Rabbit polyclonal antibody to Rag A.
Applicazioni
WB
Diluizioni consigliate
WB: 1:1,000-1:2,000
Reattività
Human, Mouse, Rat, Hamster, Xenopus
Immunogeno
Purified full length human Rag A protein (GST tag).
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare

Unconjugated

Forma del prodotto
Liquid
Formulazione
Supplied as antiserum with 0.05% Sodium Azide.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
Adenovirus E3 14.7 kDa-interacting protein 1, FIP-1, RagA, Ras-related GTP-binding protein A, RRAGA, RRAGA_HUMAN
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western blot analysis of RagA protein in the whole cell extracts (HeLa cell g). Anti-RagA antiserum was used at 1,000 dilution.Clicca sull'immagine per la visualizzazione estesa
Figure 1: Anti-Rag A Antibody (71-020)
Anti-Rag A Antibody
Western blot analysis of RagA protein in the whole cell extracts. 1. HeLa (humans) 2. MCF7 (human) 3. MOLT4 (human) 4. NIH3T3 (mouse) Proteins in the extracts (20 µg) were separated on 12.5% SDS-PAGE. Anti-RagA antibody was used at 1/1,000 dilution?Anti-rabbit IgG antibody conjugated with HRP (Abcam;ab97051) was used as the 2nd antibody at 1/10,000 dilutionClicca sull'immagine per la visualizzazione estesa
Figure 2: SDS-PAGE - Anti-Rag A Antibody (71-020)
Anti-Rag A Antibody
Immunofluorescence staining of RagA protein in HeLa cells with anti-Rag antibody. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.5% TritonX 100 and reacted with anti-RagA antibody at 1/100 dilution (A) or at 1/1,000 dilution (B). As the 2nd antibody, anti-rabbit IgG antibody conjugated with Alexa Fluor 647 was used at 1/1,000 dilution. DNA was stained with DAPI and the merged image was shown on rightClicca sull'immagine per la visualizzazione estesa
Figure 3: Immunofluorescence - Anti-Rag A Antibody (71-020)
Anti-Rag A Antibody

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