Anti-Raf1 (phospho Ser642) Anticorpo (A283073)

Anti-Raf1 (phospho Ser642) Antibody

Rabbit polyclonal antibody to Raf1 (phospho Ser642).

This antibody recognises Raf-1, also known as RAF proto-oncogene serine/threonine-protein kinase and proto-oncogene c-RAF (cRaf), when phosphorylated at serine 642. Raf-1is a ubiquitously expressed ~74 kDa member of the serine/threonine protein kinase family. Raf-1 is a MAP kinase kinase kinase and participates in the transduction of mitogenic signals from receptors on the cell membrane to the nucleus. Raf-1 is activated through phosphorylation by Ras and in turn phosphorylates MEK which activates ERK. ERK when activated regulates the expression of genes involved in cell cycle, differentiation, migration and apoptosis. As such Raf-1 is an important clinical target in cancer.Raf-1 activity can be downregulated by phosphorylation at serine residues 301 and 642.

WB

WB: 1:1,000

Rat, Human, Bovine, Chicken, Mouse, Dog, Primate

Phosphopeptide corresponding to animo acid sequence surrounding phosphorylated Ser642 of rat Raf-1.

Rabbit

Polyclonal

IgG

Unconjugated

Lot Specific

Approximately 74 kDa.

Supplied in 10mM HEPES, pH 7.5, with 150mM NaCl, 0.01% BSA, 50% Glycerol, and 0.05% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rabbit anti Rat Raf-1 (pSer642) antibody recognizes Raf-1, also known as RAF proto-oncogene serine/threonine-protein kinase and proto-oncogene c-RAF (cRaf), when phosphorylated at serine 642. Raf-1is a ubiquitously expressed ~74 kDa member of the serine/threonine protein kinase family. Raf-1 is a MAP kinase kinase kinase and participates in the transduction of mitogenic signals from receptors on the cell membrane to the nucleus. Raf-1 is activated through phosphorylation by Ras and in turn phosphorylates MEK which activates ERK. ERK when activated regulates the expression of genes involved in cell cycle, differentiation, migration and apoptosis. As such Raf-1 is an important clinical target in cancer.Raf-1 activity can be downregulated by phosphorylation at serine residues 301 and 642.

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