Anti-Raf1 (phospho Ser621) Antibody [ARC1556] (A308602)

Name: Anti-Raf1 (phospho Ser621) Antibody [ARC1556]
Brand: Antibodies.com
SKU: A308602
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC1556] antibody to Raf1 (phospho Ser621) for WB.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Raf1 (phospho Ser621) Antibody [ARC1556]

Description

Rabbit monoclonal [ARC1556] antibody to Raf1 (phospho Ser621).

Applications

Dilutions

WB: 1:500-1:2,000

Reactivity

Human, Rat

Immunogen

A synthetic phosphorylated peptide around S621 of human Phospho-Raf1-S621 (P04049).

Sequence

SASEP

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1556

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

74 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

cRaf, Proto-oncogene c-RAF, RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-Raf1 (phospho Ser621) Antibody [ARC1556] (A308602)

Western blot analysis of extracts of C6 cells, using Anti-Raf1 (phospho Ser621) Antibody [ARC1556] (A308602) at 1:1,000 dilution. C6 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Western Blot - Anti-Raf1 (phospho Ser621) Antibody [ARC1556] (A308602)

Western blot analysis of extracts of HeLa cells, using Anti-Raf1 (phospho Ser621) Antibody [ARC1556] (A308602) at 1:1,000 dilution. HeLa cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

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